Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III.
Identifieur interne : 000275 ( Main/Exploration ); précédent : 000274; suivant : 000276Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III.
Auteurs : RBID : pubmed:23463906English descriptors
- KwdEn :
- Aluminum, Animals, Collagen Type I (metabolism), Collagen Type III (metabolism), Diabetes Mellitus, Experimental, Gallium, Indium, Laser Therapy, Low-Level, Lasers, Semiconductor, Male, Matrix Metalloproteinase 2 (genetics), Matrix Metalloproteinase 2 (metabolism), Matrix Metalloproteinase 9 (genetics), Matrix Metalloproteinase 9 (metabolism), RNA, Messenger (metabolism), Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Skin (injuries), Wound Healing (radiation effects).
- MESH :
- chemical , genetics : Matrix Metalloproteinase 2, Matrix Metalloproteinase 9.
- chemical , metabolism : Collagen Type I, Collagen Type III, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, RNA, Messenger.
- chemical : Aluminum, Gallium, Indium.
- injuries : Skin.
- radiation effects : Wound Healing.
- Animals, Diabetes Mellitus, Experimental, Laser Therapy, Low-Level, Lasers, Semiconductor, Male, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction.
Abstract
The present study aimed to determine if LLLT restores the balance between mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9) and also the balance between collagen types I and III during the healing process of diabetic wounds. One hundred and twenty male Wistar rats were distributed in Control (untreated non-diabetic rats: UND); Laser (laser treated in non-diabetic rats: LTND); Diabetic (diabetic rats non-laser treated rats: UD); and Diabetic+ Laser (diabetic rats laser treated: DLT) groups. The diabetes model using streptozotocin efficiently induced diabetes, as demonstrated through increased levels of blood glucose. Diode laser (50 mW, 660 nm, 4 J/cm(2), 80 s) was applied a single time after scare induction. Twenty-four hours after LLLT application, rats were euthanized, the scarred areas were collected for MMP-2 and MMP-9 mRNA analysis and also for histological analysis (inflammation and types I and III collagen). The results demonstrated that scare in untreated diabetic rats significantly increased the MMP-2 and MMP-9 expression compared with that in non-diabetic rats (p < 0.05), while LLLT significantly reduced MMP-2 and MMP-9 expression compared with that in untreated diabetic rats (p < 0.05). To conclude, the results also showed that LLLT was able to alter the expression of MMP-9 as well as accelerate the production of collagen and increase the total percentage of collagen type III in diabetic animals.
DOI: 10.3109/14764172.2012.761345
PubMed: 23463906
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III.</title><author><name sortKey="Aparecida Da Silva, Andreia" uniqKey="Aparecida Da Silva A">Andreia Aparecida Da Silva</name><affiliation wicri:level="1"><nlm:affiliation>Graduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Graduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, SP</wicri:regionArea></affiliation></author><author><name sortKey="Leal Junior, Ernesto Cesar Pinto" uniqKey="Leal Junior E">Ernesto Cesar Pinto Leal-Junior</name></author><author><name sortKey="Alves, Ana Carolina Araruna" uniqKey="Alves A">Ana Carolina Araruna Alves</name></author><author><name sortKey="Rambo, Caroline Sobral" uniqKey="Rambo C">Caroline Sobral Rambo</name></author><author><name sortKey="Dos Santos, Solange Almeida" uniqKey="Dos Santos S">Solange Almeida Dos Santos</name></author><author><name sortKey="Vieira, Rodolfo P" uniqKey="Vieira R">Rodolfo P Vieira</name></author><author><name sortKey="De Carvalho, Paulo De Tarso Camillo" uniqKey="De Carvalho P">Paulo De Tarso Camillo De Carvalho</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.3109/14764172.2012.761345</idno><idno type="RBID">pubmed:23463906</idno><idno type="pmid">23463906</idno><idno type="wicri:Area/Main/Corpus">000762</idno><idno type="wicri:Area/Main/Curation">000762</idno><idno type="wicri:Area/Main/Exploration">000275</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum</term><term>Animals</term><term>Collagen Type I (metabolism)</term><term>Collagen Type III (metabolism)</term><term>Diabetes Mellitus, Experimental</term><term>Gallium</term><term>Indium</term><term>Laser Therapy, Low-Level</term><term>Lasers, Semiconductor</term><term>Male</term><term>Matrix Metalloproteinase 2 (genetics)</term><term>Matrix Metalloproteinase 2 (metabolism)</term><term>Matrix Metalloproteinase 9 (genetics)</term><term>Matrix Metalloproteinase 9 (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>Rats</term><term>Rats, Wistar</term><term>Real-Time Polymerase Chain Reaction</term><term>Skin (injuries)</term><term>Wound Healing (radiation effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Matrix Metalloproteinase 2</term><term>Matrix Metalloproteinase 9</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Collagen Type I</term><term>Collagen Type III</term><term>Matrix Metalloproteinase 2</term><term>Matrix Metalloproteinase 9</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Aluminum</term><term>Gallium</term><term>Indium</term></keywords><keywords scheme="MESH" qualifier="injuries" xml:lang="en"><term>Skin</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Wound Healing</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diabetes Mellitus, Experimental</term><term>Laser Therapy, Low-Level</term><term>Lasers, Semiconductor</term><term>Male</term><term>Rats</term><term>Rats, Wistar</term><term>Real-Time Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study aimed to determine if LLLT restores the balance between mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9) and also the balance between collagen types I and III during the healing process of diabetic wounds. One hundred and twenty male Wistar rats were distributed in Control (untreated non-diabetic rats: UND); Laser (laser treated in non-diabetic rats: LTND); Diabetic (diabetic rats non-laser treated rats: UD); and Diabetic+ Laser (diabetic rats laser treated: DLT) groups. The diabetes model using streptozotocin efficiently induced diabetes, as demonstrated through increased levels of blood glucose. Diode laser (50 mW, 660 nm, 4 J/cm(2), 80 s) was applied a single time after scare induction. Twenty-four hours after LLLT application, rats were euthanized, the scarred areas were collected for MMP-2 and MMP-9 mRNA analysis and also for histological analysis (inflammation and types I and III collagen). The results demonstrated that scare in untreated diabetic rats significantly increased the MMP-2 and MMP-9 expression compared with that in non-diabetic rats (p < 0.05), while LLLT significantly reduced MMP-2 and MMP-9 expression compared with that in untreated diabetic rats (p < 0.05). To conclude, the results also showed that LLLT was able to alter the expression of MMP-9 as well as accelerate the production of collagen and increase the total percentage of collagen type III in diabetic animals.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23463906</PMID><DateCreated><Year>2013</Year><Month>07</Month><Day>31</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>05</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1476-4180</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>4</Issue><PubDate><Year>2013</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology</Title><ISOAbbreviation>J Cosmet Laser Ther</ISOAbbreviation></Journal><ArticleTitle>Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III.</ArticleTitle><Pagination><MedlinePgn>210-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3109/14764172.2012.761345</ELocationID><Abstract><AbstractText>The present study aimed to determine if LLLT restores the balance between mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9) and also the balance between collagen types I and III during the healing process of diabetic wounds. One hundred and twenty male Wistar rats were distributed in Control (untreated non-diabetic rats: UND); Laser (laser treated in non-diabetic rats: LTND); Diabetic (diabetic rats non-laser treated rats: UD); and Diabetic+ Laser (diabetic rats laser treated: DLT) groups. The diabetes model using streptozotocin efficiently induced diabetes, as demonstrated through increased levels of blood glucose. Diode laser (50 mW, 660 nm, 4 J/cm(2), 80 s) was applied a single time after scare induction. Twenty-four hours after LLLT application, rats were euthanized, the scarred areas were collected for MMP-2 and MMP-9 mRNA analysis and also for histological analysis (inflammation and types I and III collagen). The results demonstrated that scare in untreated diabetic rats significantly increased the MMP-2 and MMP-9 expression compared with that in non-diabetic rats (p < 0.05), while LLLT significantly reduced MMP-2 and MMP-9 expression compared with that in untreated diabetic rats (p < 0.05). To conclude, the results also showed that LLLT was able to alter the expression of MMP-9 as well as accelerate the production of collagen and increase the total percentage of collagen type III in diabetic animals.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aparecida Da Silva</LastName><ForeName>Andreia</ForeName><Initials>A</Initials><Affiliation>Graduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.</Affiliation></Author><Author ValidYN="Y"><LastName>Leal-Junior</LastName><ForeName>Ernesto Cesar Pinto</ForeName><Initials>EC</Initials></Author><Author ValidYN="Y"><LastName>Alves</LastName><ForeName>Ana Carolina Araruna</ForeName><Initials>AC</Initials></Author><Author ValidYN="Y"><LastName>Rambo</LastName><ForeName>Caroline Sobral</ForeName><Initials>CS</Initials></Author><Author ValidYN="Y"><LastName>Dos Santos</LastName><ForeName>Solange Almeida</ForeName><Initials>SA</Initials></Author><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Rodolfo P</ForeName><Initials>RP</Initials></Author><Author ValidYN="Y"><LastName>De Carvalho</LastName><ForeName>Paulo De Tarso Camillo</ForeName><Initials>Pde T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>03</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Cosmet Laser Ther</MedlineTA><NlmUniqueID>101136419</NlmUniqueID><ISSNLinking>1476-4172</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Collagen Type I</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Collagen Type III</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>CH46OC8YV4</RegistryNumber><NameOfSubstance>Gallium</NameOfSubstance></Chemical><Chemical><RegistryNumber>CPD4NFA903</RegistryNumber><NameOfSubstance>Aluminum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.24.24</RegistryNumber><NameOfSubstance>Matrix Metalloproteinase 2</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.24.35</RegistryNumber><NameOfSubstance>Matrix Metalloproteinase 9</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Aluminum</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Collagen Type I</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Collagen Type III</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Diabetes Mellitus, Experimental</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gallium</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Laser Therapy, Low-Level</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lasers, Semiconductor</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Matrix Metalloproteinase 2</DescriptorName><QualifierName MajorTopicYN="N">genetics</QualifierName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Matrix Metalloproteinase 9</DescriptorName><QualifierName MajorTopicYN="N">genetics</QualifierName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Skin</DescriptorName><QualifierName MajorTopicYN="N">injuries</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Wound Healing</DescriptorName><QualifierName MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>3</Month><Day>6</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.3109/14764172.2012.761345</ArticleId><ArticleId IdType="pubmed">23463906</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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